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is a village in La Crosse County, Wisconsin, United States. The population was 7,342 at the 2006 census. It is part of the La Crosse, Wisconsin Metropolitan Statistical Area.
Holmen contains many wooded areas, hills, and bluffs, typical of the Driftless Area, or "Coulee Region," as local residents call it. The Mississippi River passes just to the southwest of the village.
Holmen is located at (43.955330, -91.259132). According to the United States Census Bureau, the village has a total area of 3.2 square miles (8.3 km²), all of it land.