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is a city in the Eastern Panhandle region of West Virginia, United States. The city's population was 14,972 at the 2000 census; according to a 2007 Census Bureau estimate, Martinsburg's population was 16,450, making it the largest city in the Eastern Panhandle and the ninth largest municipality in West Virginia. Martinsburg is the county seat of Berkeley County and one of the core cities in the Hagerstown-Martinsburg, MD-WV Metropolitan Statistical Area. Martinsburg and Berkeley County are growing very rapidly from the influx of people moving from Washington, D.C. and Baltimore.
Martinsburg was founded in 1778 by General Adam Stephen who named it in honor of Colonel Thomas Bryan Martin, a nephew of Thomas Fairfax, 6th Lord Fairfax of Cameron.
Martinsburg was incorporated by an act of the West Virginia Legislature on March 30, 1868.